Detection of fetal HLA-DQα sequences in maternal blood: A gender-independent technique of fetal cell identification

The objective of this study was to detect fetal HLA-DQα gene sequences in maternal blood. HLA-DQα genotypes of 70 pregnant women and their partners were determined for type A1. We specifically sought couples where the father, but not the mother, had genotype A1. In 12 women, maternal blood samples were flow-sorted. Candidate fetal cells were isolated and amplified by using PCR primers specific for a paternal HLA-DQα A1 allele. Fetal HLA-DQα A1 genotype was predicted from sorted cells; amniocytes or cheek swabs were used for confirmation. Six of twelve sorted samples had amplification products indicating the presence of the HLA-DQα A1 allele; 6/12 did not. Prediction of the fetal genotype was 100 per cent correct, as determined by subsequent amplification of amniocytes or cheek swabs. We conclude that paternally inherited uniquely fetal HLA-DQα gene sequences can be identified in maternal blood. This system permits the identification of fetal cells independent of fetal gender, and has the potential for non-invasive prenatal diagnosis of paternally inherited conditions.     

Kinetic-spectrophotometric determination of free cyanide by stopped-flow reversed flow injection analysis

A stopped-flow reversed flow injection method for the determination of free cyanide is proposed. Pyridine-barbituric acid mixture is injected in the flow system as reagent to form the colour species with cyanide. The flow is stopped when the reagent zone comes in the flow cell, where absorbance-time data are collected at 580nm wavelength. The linear range of the determination is 0.1 -10μg/ml CN-. The sampling rate is 60h-1 and the relative standard deviation is 1.6% (n=16) at 5.0 μg/ml CN-1 level. With satisfactory results, the proposed method was applied to the determination of free cyanide in wastewater without sample pretreatment.     

从植被演替和抗风性研究包兰线沙坡头段人工植被稳定性

运用群落演替与风沙流因子分析相结合的方法论证了群落的稳定性。在沙坡头地区,以油蒿为主的人工群落在长期的自然演替过程中已达到与生境条件相互适应的动态稳定过程。通过沙坡头与周边地区植被演替的比较研究,表明了该地区现有植被正形成(或已形成)偏途顶极序列,该植被在相当长的时间内会稳定存在,这些演替可促使沙坡头地区的人工植被向更为稳定的人工+自然复合生态系统以及自然生态系统方向变化。此外,风洞实验结果显示,沙坡头现有主要植被和地表能够抵抗较大风沙流胁迫,已形成了能够忍耐沙尘暴的生态系统。     

浓缩型入口下排气旋风分离器流场和性能的数值计算

运用CFD方法,对浓缩型入口下排气旋风分离器内的气固两相流动进行了研究。计算中气相采用RNGk-ε湍流模型,颗粒相采用拉格朗日坐标系下的离散相模型。重点计算了分离器流场、分离效率及其阻力特性,结果表明,采用浓缩入口后分离器内流场发生明显改变,分离器上部切向速度明显提高,有利于大颗粒的分离。同时通过对不同浓缩结构旋风分离器的分离效率和阻力特性进行分析比较,为工程设计提供了依据。     

城市生活垃圾无害化处理工艺

根据武汉市金口、郭茨口两个垃圾处理场情况 ,分析了城市生活垃圾处理现状及其危害 ,阐述了生活垃圾无害化处理工艺 ,提出生活垃圾无害化处理不仅可以实现资源的持续利用 ,而且奠定了城市可持续发展的基础     

Soil and water loss in the Lancang River-Mekong River watershed (in Yunnan section, China) and its control measures

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化学发光流动注射法测定雨水中过氧化氢

用化学发光流动注射分析装置,将16孔阀连接成异步双流路,以过氧化氢-鲁米诺-钴(Ⅱ)为化学发光体系,建立了快速测定雨水中过氧化氢的方法,最低检测限为0.1ng/ml,在0.1—400ppb范围内有良好的线性关系,相对标准差为0.78—3.03％,每小时可分析480个以上的样品.     

人工湿地处理皂素废水生产性试验研究

黄姜是生产“药用黄金”——皂素的主要原材料，生产过程中会产生大量的工业废水。皂素废水是一种难降解、污染物浓度高的酸性废水，目前还没有成功应用于工程实际的废水处理工艺。本文主要采用人工湿地处理经两相厌氧工艺一好氧工艺组合工艺处理之后的废水，以满足达标排放的要求。经过4个月试验研究得出如下结论：人工湿地COD平均去除率为47．79％，氨氮平均去除率为75．64％，总磷平均去除率为88．82％。水平潜流型人工湿地作为皂素废水深度处理工艺是可行的。     

INCREASED BASEFLOW IN IOWA OVER THE SECOND HALF OF THE 20TH CENTURY1

ABSTRACT: Historical trends in annual discharge characteristics were evaluated for 11 gauging stations located throughout Iowa. Discharge records from nine eight‐digit hydrologic unit code (HUC‐8) watersheds were examined for the period 1940 to 2000, whereas data for two larger river systems (Cedar and Des Moines Rivers) were examined for a longer period of record (1903 to 2000). In nearly all watersheds evaluated, annual base flow, annual minimum flow, and the annual base flow percentage significantly increased over time. Some rivers also exhibited increasing trends in total annual discharge, whereas only the Maquoketa River had significantly decreased annual maximum flows. Regression of stream discharge versus precipitation indicated that more precipitation is being routed into streams as base flow than as storm flow in the second half of the 20th Century. Reasons for the observed stream flow trends are hypothesized to include improved conservation practices, greater artificial drainage, increasing row crop production, and channel incision. Each of these reasons is consistent with the observed trends, and all are likely responsible to some degree in most watersheds.     

ECONOMIC COSTS AND BENEFITS OF INSTREAM FLOW PROTECTION FOR ENDANGERED SPECIES IN AN INTERNATIONAL BASIN1

ABSTRACT: The U.S. Endangered Species Act (ESA) restricts federal agencies from carrying out actions that jeopardize the continued existence of any endangered species. The U.S. Supreme Court has emphasized that the language of the ESA and its amendments permits few exceptions to the requirement to give endangered species the highest priority. This paper estimates economic costs associated with one measure for increasing instream flows to meet critical habitat requirements of the endangered Rio Grande silvery minnow. Impacts are derived from an integrated regional model of the hydrology, economics, and institutions of the upper Rio Grande Basin in Colorado, New Mexico, Texas, and Mexico. One proposal for providing minimum streamflows to protect the silvery minnow from extinction would provide guaranteed year round streamflows of at least 50 cubic feet per second in the San Acacia reach of the upper Rio Grande. These added flows can be accomplished through reduced surface diversions by New Mexico water users in dry years when flows would otherwise be reduced below the critical level required by the minnow. Based on a 44‐year simulation of future inflows to the basin, we find that some agricultural users suffer damages, but New Mexico water users as a whole do not incur damages from a policy that reduces stream depletions sufficiently to provide habitat for the minnow. The same policy actually benefits downstream users, producing average annual benefits of over $200,000 per year for west Texas agriculture, and over $1 million for El Paso municipal and industrial water users, respectively. Economic impacts of instream flow deliveries for the minnow are highest in drought years.